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basic fibroblast growth factor bfgf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fibroblast+growth+factor+2/Human+FGF-2%2C+premium+grade/pm42336896-211-24-29
    Average 95 stars, based on 150 article reviews
    basic fibroblast growth factor bfgf - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: αCT1 peptide sensitizes glioma cells to temozolomide in a glioblastoma organoid platform.
    Article Snippet: .. Cells were cultured in suspension at 37°C at 5% CO2 in Neurobasal media (minus phenol red; Gibco) with added B27 (minus Vitamin A; Gibco), human fibroblast growth factor‐2 (10 ng/ml; Miltenyi Biotec), human epidermal growth factor (10 ng/ml; Miltenyi Biotec), L‐glutamine (2mM; Gibco), sodium pyruvate (1mM; Gibco), and penicillin/ streptomycin (100 IU/ml/100 μg/ml; Gibco). .. Before seeding within hydrogels, cell aggregates are dissociated into a single‐cell suspension using TrypLE (Gibco).

    Article Title: Glial D-serine modulates oligodendrocyte lineage progression under inflammatory conditions
    Article Snippet: CD140a + cells (OPCs) retained during positive magnetic sorting were seeded at a density of 20,000 cells per well in 24-well plates onto sterile glass coverslips previously coated overnight with 0.01% poly-L-lysine (Sigma-Aldrich) at 37 °C. .. OPCs were cultured in MACS Neuro Medium (Miltenyi Biotec) supplemented with 2% MACS NeuroBrew®-21 (Miltenyi Biotec), 1% penicillin–streptomycin (Gibco, Cat. No. 15070-063), 0.5 mM GlutaMAXTM (Gibco, Cat. No. 35050-061), 10 ng/mL human platelet-derived growth factor AA (PDGF-AA; Miltenyi Biotec, Cat. No. 130-093-977), and 10 ng/mL human fibroblast growth factor-2 (FGF-2; Miltenyi Biotec, Cat. No. 130-093-837). ..

    Article Title: αCT1 peptide sensitizes glioma cells to temozolomide in a glioblastoma organoid platform
    Article Snippet: .. Cells were cultured in suspension at 37°C at 5% CO 2 in Neurobasal media (minus phenol red; Gibco) with added B27 (minus Vitamin A; Gibco), human fibroblast growth factor-2 (10 ng/ml; Miltenyi Biotec), human epidermal growth factor (10 ng/ml; Miltenyi Biotec), l -glutamine (2 mM; Gibco), sodium pyruvate (1 mM; Gibco), and penicillin/streptomycin (100 IU/ml/100 μg/ml; Gibco). .. Before seeding within hydrogels, cell aggregates are dissociated into a single-cell suspension using TrypLE (Gibco).

    Article Title: Improving Localized Radiotherapy for Glioblastoma via Small Molecule Inhibition of KIF11.
    Article Snippet: .. Cells were cultured at 37 ◦C at 5% CO2 in Neurobasal media (minus phenol red; Gibco, Grand Island, NY, USA) supplemented with B27 (minus Vitamin A; Gibco), human fibroblast growth factor-2 (10 ng/mL; Miltenyi Biotec, Bergisch Gladbach, Germany), human epidermal growth factor (10 ng/mL; Miltenyi Biotec), L-glutamine (2 mM; Gibco), sodium pyruvate (1 mM; Gibco), and penicillin/streptomycin (100 I.U./mL/100 μg/mL; Gibco). .. Cells plated adherently were on Geltrex LDEV-Free hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco), whereas in vivo studies were performed with cells grown in suspension as tumorspheres before dissociation and cell counting prior to implantation.

    Article Title: Improving Localized Radiotherapy for Glioblastoma via Small Molecule Inhibition of KIF11
    Article Snippet: .. Cells were cultured at 37 °C at 5% CO 2 in Neurobasal media (minus phenol red; Gibco, Grand Island, NY, USA) supplemented with B27 (minus Vitamin A; Gibco), human fibroblast growth factor-2 (10 ng/mL; Miltenyi Biotec, Bergisch Gladbach, Germany), human epidermal growth factor (10 ng/mL; Miltenyi Biotec), L-glutamine (2 mM; Gibco), sodium pyruvate (1 mM; Gibco), and penicillin/streptomycin (100 I.U./mL/100 μg/mL; Gibco). .. Cells plated adherently were on Geltrex LDEV-Free hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco), whereas in vivo studies were performed with cells grown in suspension as tumorspheres before dissociation and cell counting prior to implantation.

    Article Title: Isobavachalcone reduces blood glucose and promotes muscle development via dipeptidyl peptidase-4 inhibition.
    Article Snippet: Caco-2 cells (ATCC, Manassas, VA, USA) were cultured in growth media (MEM; Cytiva, Marlborough, MA, USA) + 10 % fetal bovine serum (FBS; Cytiva) + 1 % P/S (penicillin–streptomycin; Cytiva) + 1 % GlutaMax (Thermo Fisher Scientific, Waltham, MA, USA) + MEM NonEssential Amino Acids solution (Thermo Fisher Scientific)) in a humidified 5 % CO2 incubator at 37 ◦C. .. Human skeletal muscle cells (HSkMCs; ATCC, Virginia, VA, USA) were cultured in growth media (Ham's F10 (Cytiva) + 20 % FBS + 1 % P/S + 5 ng/mL human fibroblast growth factor 2 (FGF2; Miltenyi Biotec, Bergisch Gladbach, Germany)) in a humidified 5 % CO2 incubator at 37 ◦C. .. When the HSkMC reached a confluency of 100 %, the growth media were replaced with differentiation media (Dulbecco's modified Eagle medium (DMEM); (Cytiva) + 2 % FBS + 1 % P/S).

    Suspension:

    Article Title: αCT1 peptide sensitizes glioma cells to temozolomide in a glioblastoma organoid platform.
    Article Snippet: .. Cells were cultured in suspension at 37°C at 5% CO2 in Neurobasal media (minus phenol red; Gibco) with added B27 (minus Vitamin A; Gibco), human fibroblast growth factor‐2 (10 ng/ml; Miltenyi Biotec), human epidermal growth factor (10 ng/ml; Miltenyi Biotec), L‐glutamine (2mM; Gibco), sodium pyruvate (1mM; Gibco), and penicillin/ streptomycin (100 IU/ml/100 μg/ml; Gibco). .. Before seeding within hydrogels, cell aggregates are dissociated into a single‐cell suspension using TrypLE (Gibco).

    Article Title: αCT1 peptide sensitizes glioma cells to temozolomide in a glioblastoma organoid platform
    Article Snippet: .. Cells were cultured in suspension at 37°C at 5% CO 2 in Neurobasal media (minus phenol red; Gibco) with added B27 (minus Vitamin A; Gibco), human fibroblast growth factor-2 (10 ng/ml; Miltenyi Biotec), human epidermal growth factor (10 ng/ml; Miltenyi Biotec), l -glutamine (2 mM; Gibco), sodium pyruvate (1 mM; Gibco), and penicillin/streptomycin (100 IU/ml/100 μg/ml; Gibco). .. Before seeding within hydrogels, cell aggregates are dissociated into a single-cell suspension using TrypLE (Gibco).

    Recombinant:

    Article Title: Fibroblast-driven MMP-9/TIMP-1 imbalance in bronchoalveolar lavage reflects fibrotic progression in interstitial lung disease.
    Article Snippet: They were cultured and expanded in vitro in RPMI- 1640 medium supplemented with 10% fetal calf serum (FCS) and 100 U/mL penicillin–streptomycin (Euroclone, Milan, Italy), at 37°C in a humidified atmosphere containing 5% CO2. .. 21 Recombinant human fibroblast growth factor- 2 (FGF2, Miltenyi Biotech GmbH, Bergisch- Gladbach, Germany) was added at a sub- optimal concentration of 3 ng/mL, starting 1 day after plating and re- added once a week. .. When fibroblasts reached confluence, cells were detached with trypsin (Euroclone) and replated in new wells or flasks for further expansion.

    Concentration Assay:

    Article Title: Fibroblast-driven MMP-9/TIMP-1 imbalance in bronchoalveolar lavage reflects fibrotic progression in interstitial lung disease.
    Article Snippet: They were cultured and expanded in vitro in RPMI- 1640 medium supplemented with 10% fetal calf serum (FCS) and 100 U/mL penicillin–streptomycin (Euroclone, Milan, Italy), at 37°C in a humidified atmosphere containing 5% CO2. .. 21 Recombinant human fibroblast growth factor- 2 (FGF2, Miltenyi Biotech GmbH, Bergisch- Gladbach, Germany) was added at a sub- optimal concentration of 3 ng/mL, starting 1 day after plating and re- added once a week. .. When fibroblasts reached confluence, cells were detached with trypsin (Euroclone) and replated in new wells or flasks for further expansion.

    Magnetic Cell Separation:

    Article Title: Glial D-serine modulates oligodendrocyte lineage progression under inflammatory conditions
    Article Snippet: CD140a + cells (OPCs) retained during positive magnetic sorting were seeded at a density of 20,000 cells per well in 24-well plates onto sterile glass coverslips previously coated overnight with 0.01% poly-L-lysine (Sigma-Aldrich) at 37 °C. .. OPCs were cultured in MACS Neuro Medium (Miltenyi Biotec) supplemented with 2% MACS NeuroBrew®-21 (Miltenyi Biotec), 1% penicillin–streptomycin (Gibco, Cat. No. 15070-063), 0.5 mM GlutaMAXTM (Gibco, Cat. No. 35050-061), 10 ng/mL human platelet-derived growth factor AA (PDGF-AA; Miltenyi Biotec, Cat. No. 130-093-977), and 10 ng/mL human fibroblast growth factor-2 (FGF-2; Miltenyi Biotec, Cat. No. 130-093-837). ..

    Infection:

    Article Title: Hybrid regulatory elements
    Article Snippet: After infection, cells were maintained in Creative Biorray SuperCult® Skeletal Muscle Cell Growth Medium Kit (Creative Bioarray) supplemented with 10% fetal bovine serum and human fibroblast growth factor-2 (FGF-2, Miltenyi Biotec). .. After infection, cells were maintained in Creative Biorray SuperCult® Skeletal Muscle Cell Growth Medium Kit (Creative Bioarray) supplemented with 10% fetal bovine serum and human fibroblast growth factor-2 (FGF-2, Miltenyi Biotec). ..



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